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Table 1 Effect of pdhR deletion and overexpression on growth

From: More than just a metabolic regulator - elucidation and validation of new targets of PdhR in Escherichia coli

Strain

LB, μ [h-1]

MM glucose μ [h-1]

MM glycerol μ [h-1]

MM acetate μ [h-1]

MM pyruvate μ [h-1]

LJ110

1.29 +/- 0.01

0.59 +/- 0.07

0.42 +/- 0.01

0.17 +/- 0.02

0.21 +/- 0.07

LJ110ΔpdhR

1.25 +/- 0.06

0.20 +/- 0.019

0.19 +/- 0.02

0.07 +/- 0.02

0.22 +/- 0.00

LJ110/pTM30

1.28 +/- 0.02

0.60 +/- 0.00

0.41 +/- 0.01

0.14 +/- 0.03

0.20 +/- 0.02

LJ110/pTM30PdhRhis

1.26 +/- 0.01

0.53 +/- 0.00

0.43 +/- 0.01

0.13 +/- 0.03

0.19 +/- 0.01

  1. Strains with different genotypes regarding pdhR were grown in rich medium (LB) and minimal medium (MM) supplied with glucose, glycerol, acetate or pyruvate as carbon source. The growth rate was determined in the mid logarithmic phase.