Figure 2

Experimentally obtained Dynamic Dose-Responses. (A) NF-κB in the nucleus after 1 ng/ml and 10 ng/ml TNF for 30 minute pulse or continuous stimulation, as shown by EMSA at different time points. Oct-1 served as loading control. (B) Caspase-3 activity assay for continuous stimulation of 1 ng/ml TNF (solid lines) measured 4-8 h post stimulus. Control experiments without TNF (magenta) and with the caspase inhibitor Z-VAD-fmk (dashed lines). (C) and (D) Cell viability over time for 10 different TNF concentrations (0-100 ng/ml TNF). The surfaces connect the measured data points. Continuous (C) and 30 minute pulse (D) stimulations, both including error bars from three independent experiments. The isoclines (lines connecting points of same cell viability) are shown projected onto the 0% planes.