Figure 5

Sensitivity and Heterogeneity Analysis of Wild Type and NF-κB Knockout Model. (A) - (B) Global sensitivity measures defined as the slope of the linear correlation of time of death versus each population parameters, obtained using robust linear regression on a log-log plot. Dots: cell population sensitivity, stars: sensitivity of the nominal cell. The color coding highlights the slope value: the more red the higher the magnitude of the sensitivity, and the vertical black lines connect cell population and nominal cell sensitivities of the same parameter. (A) continuous stimulation, (B) 30 minutes pulse stimulation. (C) - (D) Cell viability as a function of time for different continuous stimulation of TNF concentrations (1, 3, 10, 30 ng/ml). (C) Solid lines: wild type cell population, dashed lines: without activation of the NF-κB-pathway. (D) Box plots of the distribution of the time of death within the cell population for different TNF concentrations. The solid red line depicts the median values of the cell populations; blue box: second and third quartiles; dashed line: whiskers with a maximum length of 1.5 interquartile range; red pluses: outliers. White background: wild type cells, grey background: cells without NF-κB activation.