Reaction scheme for EGFR signal transduction pathway in H1299 cells. The reaction steps of the EGFR signaling pathway are labeled from 1 to 27. These numbers correspond to the biochemical reactions in Additional file 1, Table S1. Steps 4, 11, and 15-27 are described by Michaelis-Menten kinetics and the other reactions by mass action. The individual reaction equations, the estimated parameters, and the estimated ranges are explained in Additional file 1, Table S2-5. The inhibitory effects of Mig6 and gefitinib are indicated by red lines. E11: EGFR homodimer; ShcP: free phosphorylated Shc; E11P: phosphorylated EGFR homodimer; Raf1A: activated Raf1; MEKP: singly phosphorylated MEK; MEKPP: doubly phosphorylated MEK; ERKP: singly phosphorylated ERK; ERKPP: doubly phosphorylated ERK.