EGF-dose dependent phosphorylation of signaling molecules. Phosphorylation of EGFR, Shc, MEK, and ERK in H1299EGFR-WT (green circle) and H1299L858R (red square) cells at 5 minutes after administration of the indicated concentrations of EGF. Western blots were performed twice for each protein, and the mean values are displayed together with standard deviations. Representative western blot images are shown in Additional file 1, Figure S1B. Experimental results are shown as symbol plots. Simulation results are shown as solid lines. The values were normalized for each protein so that the maximum values were equal to 1.