Figure 7
From: A general framework for modeling growth and division of mammalian cells
Numbers of replication complex (RC) over two cell divisions. RC (consisting of ORC1, Cdc6, etc.) is induced by E2F, accumulates in the nucleus, and binds DNA at 15,000 replication origins. RC is licensed by cyc/Cdk, primarily cycD/Cdk4or6 and cycE/Cdk2, after which RC is bound and traversed by DNA polymerase during DNA replication. Once traversed, the RC is released from the DNA and ubiquitinated, primarily by SCF(Skp2). The figure also shows a modeling construct, RC-count, which is used to set and maintain the 15,000-replication-orgin limit.