Skip to main content

Table 1 Impact of biomass and virus synthesis information on the estimation of Spodoptera frugiperda's post-infection metabolism

From: Hybrid metabolic flux analysis: combining stoichiometric and statistical constraints to model the formation of complex recombinant products

  Complete model W/o μ W/o vBac W/o biomass reactions W/o viral reactions
Fluxome var. a - 12.2 32.5 370.2 0.0
μ b 8.6 8.9 8.0 - 8.6
vBac c 920.5 920.5 3995861.2 920.6 -
Redundancies d 4 3 3 4 4
h/χ 2 e 0.3/7.8 0.0/7.8 0.0/7.8 102.0/9.5 0.3/9.5
  1. aFluxome variation (%), calculated as the average relative variation of all intracellular fluxes estimated by the model, excluding biosynthetic reactions for biomass or virus formation.
  2. bAdjusted (underlined) or estimated specific biomass formation rate (h-1 × 1000).
  3. cAdjusted (underlined) or estimated specific viral synthesis rate (103 infectious particles × (106 cells × h)-1).
  4. dThe number of redundancies in an overdetermined model corresponds to flux measurements in excess of the degrees of freedom of the system (see Methods).
  5. eA consistency index (h) that is lower than the corresponding χ2 (95%) value indicates consistency between flux measurements and the assumed network stoichiometry in each case (see Methods).