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Table 2 Experimental cultures used for model establishment

From: Hybrid metabolic flux analysis: combining stoichiometric and statistical constraints to model the formation of complex recombinant products

# Virus CCIa Culture system Supplementation/Treatmentb Productivityc References
1   1   - 1420.7  
2   3   - 117.0  
3   3   Gln 174.3  
4   3   Amino acids mix 177.1  
5 Ac-VP39EGFP 3 Spinner vessel IMS 227.3 [27]
6   3   Pyr 644.2  
7   3   α-KG 920.5  
8   3   Pyr/α-KG 462.4  
9   3   Pyr (24 mM) 455.2  
10     - 23.2  
11 Ac-VP39EGFP 3 Shake flask - 29.1 This work
12     PBS (50%) 0.9  
13     AICAR 3.1  
  1. In every case, cells were grown to the appropriate cell density in SF900II serum-free medium and infected with 0.1 infectious particles per cell.
  2. aCell concentration at infection (106 cells × mL-1); "1" and "3" stand for 1-1.5 and 3-4 × 106 cells × mL-1, respectively.
  3. bNutrients supplementation and culture treatment were performed as described in Methods (see indicated references for details). IMS - complete insect medium supplement.
  4. cMeasured specific viral synthesis rate (103 infectious particles × (106 cells × h)-1).