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Table 2 Experimental cultures used for model establishment

From: Hybrid metabolic flux analysis: combining stoichiometric and statistical constraints to model the formation of complex recombinant products

#

Virus

CCIa

Culture system

Supplementation/Treatmentb

Productivityc

References

1

 

1

 

-

1420.7

 

2

 

3

 

-

117.0

 

3

 

3

 

Gln

174.3

 

4

 

3

 

Amino acids mix

177.1

 

5

Ac-VP39EGFP

3

Spinner vessel

IMS

227.3

[27]

6

 

3

 

Pyr

644.2

 

7

 

3

 

α-KG

920.5

 

8

 

3

 

Pyr/α-KG

462.4

 

9

 

3

 

Pyr (24 mM)

455.2

 

10

   

-

23.2

 

11

Ac-VP39EGFP

3

Shake flask

-

29.1

This work

12

   

PBS (50%)

0.9

 

13

   

AICAR

3.1

 
  1. In every case, cells were grown to the appropriate cell density in SF900II serum-free medium and infected with 0.1 infectious particles per cell.
  2. aCell concentration at infection (106 cells × mL-1); "1" and "3" stand for 1-1.5 and 3-4 × 106 cells × mL-1, respectively.
  3. bNutrients supplementation and culture treatment were performed as described in Methods (see indicated references for details). IMS - complete insect medium supplement.
  4. cMeasured specific viral synthesis rate (103 infectious particles × (106 cells × h)-1).