Tyrosine phosphorolation pattern in ejaculated and in capacitated spermatozoa. Western Blot analysis of tyrosine phosphorylation pattern in total lysate of freshly ejaculated male gametes (T0) or in spermatozoa incubated under control condition (CTR) or in the presence of CD (CD). The arrows indicate the capacitation-related changes in P-Tyr. Brain proteins were used as positive control. The filter was normalized on the Tubulin expression. The data shown were representative of three independent experiments.