Capacitation-dependent PLCγ1 relocalization. Western Blot analysis of PLCγ1 localization in cytosolic and membrane fractions of freshly ejaculated male gametes (T0) or in spermatozoa incubated under control condition (CTR) or in the presence of CD (CD). The data showing the capacitation-dependent translocation of PLC-γ1 (arrows) from cytosol to membrane. Brain proteins were used as positive control. The filter was normalized on Tubulin expression. The data shown were representative of four independent experiments.