Reactions | Weight | Source and/or comment |
---|---|---|
Basal transcription/translation of condition or transcription factor dependent genes | 0.01 | Should be significantly lower than regulated expression |
Active Degradation | 5 | Should be significantly higher than the production |
Aft1/2p::nucleus = [Glt1/Aco1p-FeS::c, Grx3p::nucleus] ⇒ Aft1/2p::c | 10 | [73] |
Low affinity Fet4p metal transport | 0.1 | [74] |
Glutathione/Cysteine mitochondrial import | 0.1 | Balance of flux between compartments in wt |
Neoglucogenesis enzymatic reactions | 0.001 | [75] |
Reaction catalysed by Zwf1p | 10 | Major source of NADPH. [76] |
TCA cycle (reverse) | 0.1 | [77] |
Atp production by Atp-synthase | 5 | [78] |
O2m::c = [Sod1p-Cu-Zn::c] ⇒ H2O2::c | 100 | [79] |
O2m::m = [Sod2p::m] ⇒ H2O2::m | 100 | [79] |
O2m::(m or c) + H2O2::(m or c) ⇒ Ot::(m or c) | 0.2 | [79] |
O2m::(m or c) + H::(m or c) ⇒ H2O2::(m or c) | 0.1 | [79] |
Ot::(m or c) + Aco1p-FeS::(m or c) ⇒ Aco1p::(m or c) | 0.1 | Less probable than aconitase reaction but more probable than spontaneous degradation. |
O2m::(m or c) + Aco1p-FeS::(m or c ⇒ Aco1p::(m or c) | 0.1 | Same as "Ot::(m or c) + Aco1p-FeS::(m or c)". |
Ot::(m or c) + any iron-sulphur cluster containing protein ⇒ protein without the iron-sulphur cluster | 0.05 | Less probable than the same reaction involving Aco1p-FeS |
O2m::(m or c) + any iron-sulphur cluster containing protein ⇒ protein without the iron-sulphur cluster | 0.05 | Less probable than the same reaction involving Aco1p-FeS |
Ot::c + Met5p-siroheme-FeS::c ⇒ Met5p-siroheme::c | 0.01 | Less probable than other FeS proteinsdue to presence of siroheme |
O2m::c + Met5p-siroheme-FeS::c ⇒ Met5p-siroheme::c | 0.01 | Less probable than other FeS proteins due to presence of siroheme |
H2O2::c = [Ctt1p-Heme::c] ⇒ O2 | 10 | Higher turnover number than most enzymes but less than SODs [80] |
H2O2::m = [Cta1p-Heme::m] ⇒ O2 | 10 | Higher turnover number than most enzymes but less than SODs [80] |
#O2::ext ⇒ #O2::ext + O2 | 10 | Provides saturating oxygen. |
#glucose::ext ⇒ #glucose::ext + glucose::c | 0.1 | Limited glucose uptake as described in [36] |
Degradation of protein or peptide elements | 0.01 | Must be significantly less than the default. |
Degradation of other elements | 0.001 | Less than proteins degradation. |