The interaction map-guided approach improves hit rate in an RNAi screen and identifies novel cell cycle regulators. (A) Hit rate for the different classes of genes in the screen. Hit rate was determined as the percentage of genes that scored as a hit from the total number of genes tested for that class. (B) COP9 signalosome interaction map from Figure 1B showing genes identified as hits in the current study (yellow). These included a number of genes not previously identified as hits in RNAi screens for cell cycle regulators (yellow circles).