Figure 5

Ranalexin exposure induces sensitivity to hypo-osmotic stress. Cells of S. aureus RN4220 were exposed to sub-lethal concentrations of ranalexin (10 μg ml^-1; ♦); vancomycin (0.8 μg ml^-1; ▲); ranalexin + vancomycin (10 μg ml^-1 + 0.8 μg ml^-1; ●) in TSB, compared with untreated cells (■) and untreated cells of the vraR mutant in TSB (□). After 30 min incubation in TSB cells were harvested, washed twice with distilled water to remove the antimicrobials and resuspended in distilled water. Viable counts were determined throughout the experiment. Error bars represent the standard error of the mean (n = 3).