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Table 3 Assessment of anaerobic constraints used in idFV715

From: Expanding a dynamic flux balance model of yeast fermentation to genome-scale

 

Oxygen uptake

Sterols uptake**

Quinone reactions

Complete TCA cycle*

Time (H)

Biomass (g/L)

Glycerol (g/L)

Ethanol (g/L)

Experimental wt

-

-

-

-

122.0

5.38 ± 0.43

7.93 ± 0.282

107 ± 3.52

Model 1 (Used as wt)

off

unlimited

off

off

130.50

5.14

10.02

106.54

Model 2

off

unlimited

on

off

137.50

5.10

1.50

115.07

Model 3

off

unlimited

off

on

129.50

5.50

1.66

115.21

Model 4

off

unlimited

on

on

137.50

5.09

2.14

114.35

Model 5

on

unlimited

off

off

111.50

5.49

29.44

90.44

Model 6

off

limited

off

off

L

L

L

L

  1. Assessing the impact of different factors in anaerobic metabolism. Fermentations were carried out in triplicate in the nitrogen-limited medium MS300 at 28°C. Different models represent simulations by varying the activation or inhibition of the specific constraints. L = lethal; * Complete TCA cycle means no branches. **Sterols uptake means ergosterol and zymosterol uptake.
  2. L = lethal
  3. *Complete TCA cycle means no branches
  4. ** Sterols uptake mean ergosterol and zymosterol uptake