Time series gene expression profiling and temporal regulatory pathway analysis of BMP6 induced osteoblast differentiation and mineralization

Table 1 Interpretation and validation of the significant KEGG pathways inferred by GAGE.

KEGG pathways	Most perturbed	References	Other evidences
TGF-beta signaling pathway	8h	[5, 57]	BMP signal targets IDs, SMAD6-7, DLX5 extremely up
Cytokine-cytokine receptor interaction	8h	[33, 58]	IFN target gene sets down (Table 4)
Wnt signaling pathway	8h	[59, 60]
Jak-STAT signaling pathway	24h	[58, 61]	STAT1 target genes down
MAPK signaling pathway	24h	[22, 23]	--
p53 signaling pathway	24h	[62, 63]	--
Focal adhesion	10d	[20, 21]	--
ECM-receptor interaction	10d	[64, 65]	--

Literature findings and experimental evidence suggest regulatory roles of these KEGG pathways in BMP6 induced osteoblast differentiation and mineralization.