Table 5 Proposed essential enzymes that can be candidate drug targets for B. cenocepacia J2315

Amino acid metabolism

EC-4.2.3.4

AroB

3-dehydroquinate synthase

EC-4.2.3.5

AroC

Chorismate synthase

EC-1.1.1.25

AroE

Shikimate dehydrogenase

EC-1.3.1.26

DapB

Dihydrodipicolinate reductase

EC-2.3.1.117

DapD

Tetrahydrodipicolinate succinylase

EC-5.1.1.7

DapF

Diaminopimelate epimerase

EC-2.7.2.4

LysC

Aspartate kinase

Lipid synthesis

EC-6.4.1.2

AccA*

Acetyl-CoA carboxylase carboxyltransferase subunit-α

EC-6.4.1.2

AccB*

Acetyl-CoA carboxylase biotin carboxyl carrier protein subunit

EC-6.4.1.2

AccC*

Acetyl-CoA carboxylase biotin carboxylase subunit

EC-6.4.1.2

AccD*

Acetyl-CoA carboxylase subunit-β

EC-2.7.8.8

PssA

Phosphatidylserine synthase

Cell wall/LPS synthesis

-

ArnA1^#

UDP-Ara4N formyltransferase

-

ArnA2^#

UDP-4-keto-5-carboxypentose decarboxylase

-

ArnB^#

UDP-4-ketopentose aminotransferase

-

ArnC^#

Ara4N Und-P transferase

-

ArnT^#

Ara4N transferase

EC-3.6.1.27

BacA

Undecaprenyl pyrophosphate phosphatase

EC-2.5.1.55

KdsA

2-dehydro-3-deoxyphosphooctonate aldolase

EC-2.7.7.38

KdsB

3-deoxy-manno-octulosonate cytidylyltransferase

EC-2.3.1.129

LpxA

UDP-N-acetylglucosamine acyltransferase

EC-2.4.1.182

LpxB

Lipid-A-disaccharide synthase

EC-3.5.1.-

LpxC

UDP-3-O-[3-hydroxymyristoyl]N-acetylglucosamine deacetylase

EC-2.7.1.130

LpxK

Tetraacyldisaccharide 4'-kinase

EC-2.5.1.7

MurA*

UDP-N-acetylglucosamine 1-carboxyvinyltransferase

EC-1.1.1.158

MurB

UDP-N-acetylmuramate dehydrogenase

EC-6.3.2.8

MurC

UDP-N-acetylmuramate--L-alanine ligase

EC-6.3.2.9

MurD

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

EC-6.3.2.13

MurE

UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

EC-2.4.1.227

MurG

Undecaprenyldiphospho-muramoylpentapeptide-β-N-acetylglucosaminyltransferase

EC-1.1.1.22

Udg^#

UDP-glucose dehydrogenase

EC-2.4.1.-

WaaF

UDP-glucose:(heptosyl) LPS-α-1,3-glucosyltransferase

EC-5.1.3.13

RmlC

dTDP-4-dehydrorhamnose 3,5-epimerase

Vitamin and cofactor synthesis

EC-2.7.11.5

AceK

Bifunctional isocitrate dehydrogenase kinase/ phosphatase protein

EC-4.1.2.25

FolB

Dihydroneopterin aldolase

EC-2.5.1.15

FolP*

Dihydropteroate synthase

EC-1.2.1.70

HemA

Glutamyl-tRNA reductase

EC-2.1.2.11

PanB

3-methyl-2-oxobutanoate hydroxymethyltransferase

EC-6.3.2.1

PanC

Pantoate--β-alanine ligase

EC-1.1.1.169

PanE

2-dehydropantoate 2-reductase

EC-3.1.3.1

PhoA*

Alkaline phophatase

EC-3.5.4.25

RibB

Bifunctional 3,4-dihydroxy-2-butanone 4-phosphate synthase

EC-3.5.4.26

RibD

Riboflavin-specific deaminase/reductase

EC-2.5.1.9

RibE*

Riboflavin synthase subunit-α

EC-2.5.1.9

RibH*

6,7-dimethyl-8-ribityllumazine synase