Skip to main content

Table 5 Proposed essential enzymes that can be candidate drug targets for B. cenocepacia J2315

From: Exploring the metabolic network of the epidemic pathogen Burkholderia cenocepacia J2315 via genome-scale reconstruction

Functional subsystem EC No. Protein Enzyme name
Amino acid metabolism EC- AroB 3-dehydroquinate synthase
  EC- AroC Chorismate synthase
  EC- AroE Shikimate dehydrogenase
  EC- DapB Dihydrodipicolinate reductase
  EC- DapD Tetrahydrodipicolinate succinylase
  EC- DapF Diaminopimelate epimerase
  EC- LysC Aspartate kinase
Lipid synthesis EC- AccA* Acetyl-CoA carboxylase carboxyltransferase subunit-α
  EC- AccB* Acetyl-CoA carboxylase biotin carboxyl carrier protein subunit
  EC- AccC* Acetyl-CoA carboxylase biotin carboxylase subunit
  EC- AccD* Acetyl-CoA carboxylase subunit-β
  EC- PssA Phosphatidylserine synthase
Cell wall/LPS synthesis - ArnA1# UDP-Ara4N formyltransferase
  - ArnA2# UDP-4-keto-5-carboxypentose decarboxylase
  - ArnB# UDP-4-ketopentose aminotransferase
  - ArnC# Ara4N Und-P transferase
  - ArnT# Ara4N transferase
  EC- BacA Undecaprenyl pyrophosphate phosphatase
  EC- KdsA 2-dehydro-3-deoxyphosphooctonate aldolase
  EC- KdsB 3-deoxy-manno-octulosonate cytidylyltransferase
  EC- LpxA UDP-N-acetylglucosamine acyltransferase
  EC- LpxB Lipid-A-disaccharide synthase
  EC-3.5.1.- LpxC UDP-3-O-[3-hydroxymyristoyl]N-acetylglucosamine deacetylase
  EC- LpxK Tetraacyldisaccharide 4'-kinase
  EC- MurA* UDP-N-acetylglucosamine 1-carboxyvinyltransferase
  EC- MurB UDP-N-acetylmuramate dehydrogenase
  EC- MurC UDP-N-acetylmuramate--L-alanine ligase
  EC- MurD UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
  EC- MurE UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase
  EC- MurG Undecaprenyldiphospho-muramoylpentapeptide-β-N-acetylglucosaminyltransferase
  EC- Udg# UDP-glucose dehydrogenase
  EC-2.4.1.- WaaF UDP-glucose:(heptosyl) LPS-α-1,3-glucosyltransferase
  EC- RmlC dTDP-4-dehydrorhamnose 3,5-epimerase
Vitamin and cofactor synthesis EC- AceK Bifunctional isocitrate dehydrogenase kinase/ phosphatase protein
  EC- FolB Dihydroneopterin aldolase
  EC- FolP* Dihydropteroate synthase
  EC- HemA Glutamyl-tRNA reductase
  EC- PanB 3-methyl-2-oxobutanoate hydroxymethyltransferase
  EC- PanC Pantoate--β-alanine ligase
  EC- PanE 2-dehydropantoate 2-reductase
  EC- PhoA* Alkaline phophatase
  EC- RibB Bifunctional 3,4-dihydroxy-2-butanone 4-phosphate synthase
  EC- RibD Riboflavin-specific deaminase/reductase
  EC- RibE* Riboflavin synthase subunit-α
  EC- RibH* 6,7-dimethyl-8-ribityllumazine synase
  1. # Essential enzymes unique for B. cenocepacia J2315 (others are shared between B. cenocepacia J2315 and P. aeruginosa PAO1).
  2. * The proteins have had approved drugs from the DrugBank database.