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Table 5 Proposed essential enzymes that can be candidate drug targets for B. cenocepacia J2315

From: Exploring the metabolic network of the epidemic pathogen Burkholderia cenocepacia J2315 via genome-scale reconstruction

Functional subsystem EC No. Protein Enzyme name
Amino acid metabolism EC-4.2.3.4 AroB 3-dehydroquinate synthase
  EC-4.2.3.5 AroC Chorismate synthase
  EC-1.1.1.25 AroE Shikimate dehydrogenase
  EC-1.3.1.26 DapB Dihydrodipicolinate reductase
  EC-2.3.1.117 DapD Tetrahydrodipicolinate succinylase
  EC-5.1.1.7 DapF Diaminopimelate epimerase
  EC-2.7.2.4 LysC Aspartate kinase
Lipid synthesis EC-6.4.1.2 AccA* Acetyl-CoA carboxylase carboxyltransferase subunit-α
  EC-6.4.1.2 AccB* Acetyl-CoA carboxylase biotin carboxyl carrier protein subunit
  EC-6.4.1.2 AccC* Acetyl-CoA carboxylase biotin carboxylase subunit
  EC-6.4.1.2 AccD* Acetyl-CoA carboxylase subunit-β
  EC-2.7.8.8 PssA Phosphatidylserine synthase
Cell wall/LPS synthesis - ArnA1# UDP-Ara4N formyltransferase
  - ArnA2# UDP-4-keto-5-carboxypentose decarboxylase
  - ArnB# UDP-4-ketopentose aminotransferase
  - ArnC# Ara4N Und-P transferase
  - ArnT# Ara4N transferase
  EC-3.6.1.27 BacA Undecaprenyl pyrophosphate phosphatase
  EC-2.5.1.55 KdsA 2-dehydro-3-deoxyphosphooctonate aldolase
  EC-2.7.7.38 KdsB 3-deoxy-manno-octulosonate cytidylyltransferase
  EC-2.3.1.129 LpxA UDP-N-acetylglucosamine acyltransferase
  EC-2.4.1.182 LpxB Lipid-A-disaccharide synthase
  EC-3.5.1.- LpxC UDP-3-O-[3-hydroxymyristoyl]N-acetylglucosamine deacetylase
  EC-2.7.1.130 LpxK Tetraacyldisaccharide 4'-kinase
  EC-2.5.1.7 MurA* UDP-N-acetylglucosamine 1-carboxyvinyltransferase
  EC-1.1.1.158 MurB UDP-N-acetylmuramate dehydrogenase
  EC-6.3.2.8 MurC UDP-N-acetylmuramate--L-alanine ligase
  EC-6.3.2.9 MurD UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
  EC-6.3.2.13 MurE UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase
  EC-2.4.1.227 MurG Undecaprenyldiphospho-muramoylpentapeptide-β-N-acetylglucosaminyltransferase
  EC-1.1.1.22 Udg# UDP-glucose dehydrogenase
  EC-2.4.1.- WaaF UDP-glucose:(heptosyl) LPS-α-1,3-glucosyltransferase
  EC-5.1.3.13 RmlC dTDP-4-dehydrorhamnose 3,5-epimerase
Vitamin and cofactor synthesis EC-2.7.11.5 AceK Bifunctional isocitrate dehydrogenase kinase/ phosphatase protein
  EC-4.1.2.25 FolB Dihydroneopterin aldolase
  EC-2.5.1.15 FolP* Dihydropteroate synthase
  EC-1.2.1.70 HemA Glutamyl-tRNA reductase
  EC-2.1.2.11 PanB 3-methyl-2-oxobutanoate hydroxymethyltransferase
  EC-6.3.2.1 PanC Pantoate--β-alanine ligase
  EC-1.1.1.169 PanE 2-dehydropantoate 2-reductase
  EC-3.1.3.1 PhoA* Alkaline phophatase
  EC-3.5.4.25 RibB Bifunctional 3,4-dihydroxy-2-butanone 4-phosphate synthase
  EC-3.5.4.26 RibD Riboflavin-specific deaminase/reductase
  EC-2.5.1.9 RibE* Riboflavin synthase subunit-α
  EC-2.5.1.9 RibH* 6,7-dimethyl-8-ribityllumazine synase
  1. # Essential enzymes unique for B. cenocepacia J2315 (others are shared between B. cenocepacia J2315 and P. aeruginosa PAO1).
  2. * The proteins have had approved drugs from the DrugBank database.