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Table 1 Operating conditions of the fed-batch experiments

From: Cell functional enviromics: Unravelling the function of environmental factors

   Fed-batch 1 Fed-batch 2 Fed-batch 3 Fed-batch 4 Fed-batch 5
Initial concentration (mM) Glc i 5.5 5.7 0.18 6.2 4.8
  Gln i 0.2 0.5 0.42 3.9 0.1
  Glu i 1.6 0.6 0.82 0.6 4.0
  Ser i 2.3 1.3 1.30 1.4 1.4
  Asp i 1.4 0.8 0.65 0.8 0.8
  First feed
Started when Glc and/or Gln went below 0.15 mM
Glc = 183.0
Gln = 15.0
Glu = 20.0
Glc = 182.0
Gln = 5.2
Glu = 31.0
Glc = 150.0
Gln = 4.2
Glu = 29.2
Glc = 198.0 Glc = 183.0
Composition of feeds (mM) Second feed
Started when Ser became limiting (~75 h of operation)
- Glc = 193.0
Gln = 4.5
Glu = 36.0
Ser = 33.0
Cys = 8.5
Glc = 160.0
Gln = 5.4
Glu = 25.6
Culture medium 5 times concentrated
Glc = 156.0
Gln = 42.8
-
Flow rate Adjusted to keep glucose between 0.05-0.15 mM or/and glutamine between 0.05-0.25 mM