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Table 6 Kinetic equations for maltotriose disproportionation to glucose and maltopentaose by DPE1.

From: Kinetic modeling and exploratory numerical simulation of chloroplastic starch degradation

Haldane relation 1

[30]

Haldane relation 2

[30]

Haldane relation 3

[30]

Keq

1.0 [53]

KMA

3.3 mM [54]

Vfmax

; [DPE1] = 2 μM

KMP1

11.7 mM [55]

KMQ2

0.21 mM [55]

KiQ3

0.1 mM [55]

KiP4

5.57 mM

KiA5

746 μM

  1. Concentrations A= maltotriose, P = glucose, and Q = maltopentaose.
  2. 1 The K M (glucose) value was taken from that for the disproportionation reaction between 4-nitrophenyl-α-D-maltoheptaoside-4-6-O-ethylidene (EPS) as donor and glucose as acceptor catalyzed by the cyclodextrin glycosyltransferase (CGTase) enzyme in Thermoanaerobacterium thermosulfurigenes.
  3. 2Substituted by the T. thermosulfurigenes K M (EPS) value for the disproportionation reaction between EPS as donor and maltose as acceptor catalyzed by the T. thermosulfurigenes CGTase.
  4. 3Taken as 0.1 × the competitive K i (γ-cyclodextrin) value for the disproportionation between EPS as donor and maltose as acceptor catalyzed by T. thermosulfurigenes CGTase.
  5. 4From Haldane relations 1 and 2.
  6. 5From Haldane relation 3.
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BMC Systems Biology

ISSN: 1752-0509

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