Figure 2

miR-148a and its regulated PIN. (A) miR-148a expression levels (Mean ± standard error) were detected and normalized to U6 snRNA by qRT-PCR (P < 0.0001, paired t-test). (B) The effect of miR-148a expression levels on patient survival was measured. Cut-off value of 0.101 was determined based on the finding that miR-148a expression levels in 60% of patients (37 out of 62) that survived for a period of 2 to 140 months after surgical resection were above 0.101. Solid and dotted lines represent high (≧0.101, N = 50) and low (<0.101, N = 12) miR-148a levels. (C) The AUC of miR-148a was 0.84 (0.76-0.90, 95% confidence interval) (ROC curves analysis, P = 0.0001). (D) Red nodes represent up-regulated target genes and blue nodes represent their interacting partners within the human PIN. Green edges represent predicted interactions between miRNAs and targets and purple edges represent PPIs between proteins. (E) Nodes represent enriched GO terms in the miR-148a-regulated PIN and edges represent the relationships in the GO. P-value of each enriched GO terms: cell-matrix adhesion: 9.5619E-6, wound healing: 5.8018E-5, blood coagulation: 8.0094E-4, cell surface receptor linked signal transduction: 5.3054E-5, integrin-mediated signaling pathway: 3.1108E-6, enzyme linked receptor protein signaling pathway: 2.3001E-4.