The relationship between miR-148a and PAI-1 (P), VAV2 (V), ITGB8 (I-8) and ITGA5 (I-5). (A) White and light gray bars represent miR-148a precursor and inhibitor, respectively. Black and dark gray represent precursor and inhibitor negative controls, respectively. Over-expressed miR-148a reduces invasion (B), migration (C) and adhesion (D) of tumor cells. Invasive, migratory and adhesive activities of tumor cells were measured after transfection for 48hr with miR-148a precursor (light gray bar) and inhibitor-treated (dark gray bar) AGS, SC-M1 and MKN-45 cell lines. Precursor (white bar) and inhibitor (black bar) negative controls were used (t-test, *P < 0.05; **P < 0.01; ***P < 0.001).