Inferring transcriptional gene regulation network of starch metabolism in Arabidopsis thaliana leaves using graphical Gaussian model

Table 5 The statistics from Mann–Whitney U test of chloroplast and starch morphology, and granule number

	Arabidopsis Line	N¹	Area (μm²)	Width (μm)	Height (μm)	Circularity
CHLOROPLAST			Relative Mean Rank²
	Atidd5	259	35.52	41.96	45.06	42.75
	WT (Col-0)	368	60.33	55.80	53.61	55.24
	col	176	38.81	53.70	33.94	48.20
	WT (Col-0)		55.49	48.37	57.82	50.99
			P-value (Mann–Whitney U)³
	Atidd5		3.38E-26	3.52E-09	2.60E-04	9.86E-08
	col		2.99E-10	0.0442	1.90E-19	0.2919
STARCH GRANULE			Relative Mean Rank
	Atidd5	890	45.63	47.58	47.35	47.66
	WT (Col-0)	895	54.40	52.47	52.69	52.38
	col	505	48.82	54.72	38.91	60.53
	WT (Col-0)		50.72	47.40	56.31	44.11
			P-value (Mann–Whitney U)
	Atidd5		1.38E-10	3.45E-04	9.54E-05	5.54E-04
	col		0.2352	5.23E-06	2.49E-27	1.64E-24
GRANULE NUMBER			Number of starch granule / chloroplast
			Relative Mean Rank
	Atidd5	283	53.57
	WT (Col-0)	408	47.65
	col	177	54.18
	WT (Col-0)		48.31
			P-value (Mann–Whitney U)
	Atidd5		0.0067
	col		0.0213

1. N is a number of observed chloroplast (or starch granule) from Transmission electron micrographs. For the granule number analysis, N is a number of observed chloroplast.
2. Relative Mean Rank was calculated from a ratio of mean rank to total rank and multiplied by 100 when the data was ranked in an ascending order.
3. The P-value calculated from the Mann–Whitney U test less than 0.05 was shown in bold letters indicating a significant difference of morphological parameters between the mutant and the wild type.

ISSN: 1752-0509