Figure 4

Examples of our hypotheses that have been experimentally validated in the literature. (a) RPT4 is a knockout target of Hsf1 from Reimand et al.’s study [11]. Since the RPT4 promoter has no Hsf1 binding sites, it is hard to imagine how Hsf1 regulates RPT4. Our method identified a path Hsf1→RPN4→RPT4, suggesting Hsf1 regulates RPT4 through the intermediate TF Rpn4. The identified path has been experimentally proven to exist in the yeast cells [6, 7, 21–26]. (b) ETR1 is a knockout target of Stp2 from Reimand et al.’s study [11]. Since the ETR1 promoter has no Stp2 binding sites, it is hard to imagine how Stp2 regulate ETR1. Our method identified a path Stp2→ADR1→ETR1, suggesting Stp2 regulates ETR1 through the intermediate TF Adr1. The identified path has been experimentally proven to exist in the yeast cells [6, 7, 23, 27–29].