Designing optimal cell factories: integer programming couples elementary mode analysis with regulation

BMC Systems Biology

Table 5 List of all MCS for the regulatory BLP in Figure 1

i	Gene deletion		Reaction deletion			f _i
1	GR2	GR5a	R2	R5	R10	308.2	*
2	GR2	GR5b	R2	R5	R10	308.2	*
3	GR2	GR11	R2	R10	R11	308.2	*
4	GR2		R2	R9	R10	211.2

List of all MCS for the regulatory BLP. MCS are sorted in decreasing order of the objective function $f_{i} = w_{2}^{T} x^{(i)} + | | y^{(i)} | |$ as calculated by our algorithm. (The sequence of MCS with equal objective value may differ depending on the BLP algorithm.) * marks MCS for which full genetic information is available. MCS are split in the gene deletion part and the reaction deletion part. Note that the first three MCS require deletions of two genes. The corresponding reaction deletions are a consequence of those deletions. MCS 4 however, is not fully annotated (noticeable in the drop of f_i), and would require the deletions of genes and reactions (GR2 and R9).

ISSN: 1752-0509