Figure 10

Glycolytic oscillations in the wild type BY4743 compared to isogenic mutants in genes encoding respective components of the cAMP signal transduction pathway: BY4743 (blue trace); pde2 Δ (red trace), ira2 Δ (green trace). Oscillations were induced by the addition of glucose to a concentration of 30 mM after 60 seconds, followed by addition of KCN to a final concentration of 5 mM after 140 seconds. NADH fluorescence intensity was followed using a fluorimeter.