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Figure 5 | BMC Systems Biology

Figure 5

From: Emergence of bimodal cell population responses from the interplay between analog single-cell signaling and protein expression noise

Figure 5

Confirming the presence of negative feedback. HEK293 cells were pretreated with 5 μM U0126 or vehicle alone (DMSO) for 30 min prior to stimulation with 0.1 nM, 1 nM or 10 nM of EGF (A) or TGFα (B) for 5 or 30 minutes. Control cells were left unstimulated (−). Total cell lysates were assayed for activated RasGTP as described in “Materials and Methods” section. IP denotes the pull-down fraction, TCL denotes total cell lysate, and IB denotes immunoblot.

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