Workflow used to analyze the response of murine primary hepatocytes to the change of culture medium after a period of starvation. The workflow of the ExTILAR inference study presented here can be roughly divided into 7 single steps. After pre-processing of the the raw data (step 1), the gene expression profiles were clustered and DETFs were extracted (step 2). Over-represented TFBSs for the clusters were determined. Regulating TFs for the selected DETFs were extracted from literature knowledge (step 3). In step 4, the information of the two previous steps were pooled to extract prior-knowledge and validation knowledge. Using the expression profiles of the DETFs, the mean cluster expression profiles, the information about the regulating TFs and the prior-knowledge, ExTILAR was applied to infer a TFN (step 5). The resulting network was checked for present validation knowledge, analyzed and interpreted to extract testable hypothesis (step 6). The extracted hypotheses were validated experimentally (step 7).