Figure 6

Robust subsets of co-regulated genes presented as a bipartite graph.. We have identified high Activin along with PI3K inhibition or activin in combination with WNT3A to work the best to co-regulate early endoderm marker CER and late endoderm markers HNF6. The Group 2 TFs HNF4α and HNF6 are part of the network inducing NGN3 and PDX1, reminiscent of the pancreatic genotype and are favored by high activin with PI3KI and WNT3A.