Figure 4

Colour-pattern analysis of tungstate-induced and heat-shock-induced modifications of major eyespots on dorsal and ventral surfaces. (A, B) Dorsal (A) and ventral (B) major eyespots and PFEs of the forewing. Four individuals were aligned according to the possible time sequence of development. Treatment modes are indicated above each wing portion; light blue arrowheads indicate PFEs. Smaller eyespots and PFEs that were dislocated closer to the eyespot focus were considered to represent earlier stages of development. The open area produced by tungstate treatment on the distal sides of smaller eyespots may be attributed to repulsion from PFEs located nearby. Note the size and structural changes of the eyespots. Additionally, note the positional and width changes in the PFEs. Modified from Otaki [27]. (C) Percentages of white foci, inner black core rings, yellow rings, and outer black rings of the major eyespots in the four individuals shown in B. The distal sides of the major eyespots were measured from the centres of the focal areas. (D) Relative distances of PFEs from the focus and relative PFE widths in the four individuals shown in B. The distance was measured from the centre of the focus to the nearest part of the PFEs. The distance and width of the "no treatment" individual were adjusted to 1.00, and other data were normalised accordingly.