Figure 1

Projecting Fatty Acid  β  -oxidation from S. cerevisiaeto Y. lipolytica.

This simplified schematic view shows how the Fatty Acid β-oxidation scaffold pathway from S. cerevisiae iIN800[35] was modified to adequately describe Y. lipolytica metabolism. (a) Simplified version of fatty acid β-oxidation diagram of S. cerevisiae iIN800. (b) Fatty acid β-oxidation in the reconstructed model for Y. lipolytica, with a constitutive peroxisome compartment and cytosol ↔ peroxisome transport reactions. Species-specific transport mechanisms for long and short fatty acid chains (PXA1,2 and PEX11) are highlighted in green and blue. Long chains are activated (-CoA) before being transported to the peroxisome. Y. lipolytica can directly process Octanoic (C8), Hexanoic (C6), Butyric (C4) acid, and C18:2, so they were added to our model (in yellow). Our method predicted the family expansion of S. cerevisiae POX1/FOX1 into POX1-6, and the reduction of S. cerevisiae family FAA1-4 to FAA1 (YALI0D17864g), which modified the genome associations of most of the pathway. POX1-6 are written in order of specificity: POX2,5,4 for long chains and POX3,5,4 for short chains[42].