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Figure 8 | BMC Systems Biology

Figure 8

From: Genome-scale modeling using flux ratio constraints to enable metabolic engineering of clostridial metabolism in silico

Figure 8

FBrAtio predictions using the i CAC490 model at various levels of CoAT knockdown and AAD over-expression. Shown are predictions of growth (x10) (blue), acetone (cyan), butanol (red), and ethanol (green) production during solventogenesis (SPF = 5). The following flux ratios were applied: (i) f(rTHL):f(rAAD _1), (ii) f(rCoAT):f(rBHBD), (iii) f(rPTB): f (rAAD _2), and (iv) f(rADD _3):f(rAAD_ 1). The following were held constant: (i) glucose uptake rate of 10 m m o l h r · g D C W , (ii) constraints listed in Table 1, (iii) f(rTHL):f(rPTA) = 2, (iv) f(CO 2 export):f(CO 2 conversion) = 5, (v) f(rPFO):f(rLDH) = 10, and (vi) f(rCoAT, butyrate):f(rCoAT, acetate) = 0.63.

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