Table 1 Types of assays used to detect the PPI reported in the PPI network of the yeast peroxisome
From: From networks of protein interactions to networks of functional dependencies
Assay | PPI | % |
|---|---|---|
Binary methods | Â | Â |
Two-hybrid | 546 | 22,2 |
Biochemical Activity | 295 | 12,0 |
PCA | 194 | 7,9 |
| Â | 1,035 | 42,1 |
Cluster methods | Â | Â |
Affinity Capture-MS | 1,112 | 45,3 |
Affinity Capture-Western | 164 | 6,7 |
Co-crystal Structure | 1 | 0,0 |
Co-fractionation | 62 | 2,5 |
Co-localization | 9 | 0,4 |
Co-purification | 17 | 0,7 |
Far Western | 2 | 0,1 |
Reconstituted Complex | 55 | 2,2 |
| Â | 1,422 | 57,9 |
Total Binary and Cluster | 2,457 | 100 |