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Table 1 Types of assays used to detect the PPI reported in the PPI network of the yeast peroxisome

From: From networks of protein interactions to networks of functional dependencies

Assay PPI %
Binary methods   
Two-hybrid 546 22,2
Biochemical Activity 295 12,0
PCA 194 7,9
  1,035 42,1
Cluster methods   
Affinity Capture-MS 1,112 45,3
Affinity Capture-Western 164 6,7
Co-crystal Structure 1 0,0
Co-fractionation 62 2,5
Co-localization 9 0,4
Co-purification 17 0,7
Far Western 2 0,1
Reconstituted Complex 55 2,2
  1,422 57,9
Total Binary and Cluster 2,457 100
  1. For each assay, the PPI detections are shown as total number and percentage. The total number of PPI detections exceeds the actual number of PPI (2,457 versus 2,433), because 24 PPI were detected with two methods. See also Additional file 1 (for a list of the proteins in the PPI network) and Additional file 2 (for a visual display of the PPI network).
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