Modeling of human factor Va inactivation by activated protein C

BMC Systems Biology

Table 1 Equations describing APC inactivation of factor Va

Eqn	Chemical Expression^a	k_off	k_on	k_cat	k_dis
Eqn	Chemical Expression^a	(s^-1)	(M^-1 s^-1)	(s^-1)	(s^-1)
1	APC + FVa ← 2-1 → APCFVa	0.7	1 x 10⁸
2	APCFVa −3 → APC + FVa_i⁵⁰⁶			1.0
3	APCFVa −4 → APC + FVa_i³⁰⁶			0.064^b
3	APCFVa −4 → APC + FVa_i³⁰⁶			0.192^c
4	APC + FVa_i⁵⁰⁶ ← 2-1 → APCFVa_i⁵⁰⁶	0.7	1 x 10⁸
5	APC + FVa_i³⁰⁶ ← 2-1 → APCFVa_i³⁰⁶	0.7	1 x 10⁸
6	APCFVa_i⁵⁰⁶ -4 → APC + FVa_i^306/506			0.064^b
6	APCFVa_i⁵⁰⁶ -4 → APC + FVa_i^306/506			0.192^c
7	APCFVa_i³⁰⁶ -3 → APC + FVa_i^306/506			1.0
8	FVa_i³⁰⁶ -5 → FVa^1-306FVa^LC + FVa^307-679/709				0.028
9	FVa_i^306/506 -5 → FVa^1-306FVa^LC + FVa^307-506 + FVa^507-679/709				0.028
10	APC + FVa^1-306FVa^LC ← 2-1 → APC FVa^1-306FVa^LC	0.7	1 x 10⁸

^a For equilibrium expressions denoted by ← 2-1→, the first number listed describes the reverse reaction given in the k_off column.
^b Rate constant used for mathematical simulations as determined by inactivation studies with bovine FVa and bovine APC in Hockin et al. [33].
^c Rate constant used for mathematical simulations, as determined by inactivation studies with human FVa and human APC in this study, is 0.192 s^-1. This new rate constant will be used in all subsequent mathematical simulations.

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