Modeling suggests that gene circuit architecture controls phenotypic variability in a bacterial persistence network

Table 1 Chemical reaction parameters for the HipBA system

Reaction	Reactants	Products	Reaction Rate	Value	Units	Range Tested	Reference
transcription	P	M + P	α	60	hour^-1	6-600	[29, 30]
transcription	P'	M + P'	α_B2	6	hour^-1	0.6-60	[29, 30]
transcription	P''	M + P''	α_AB2A	6	hour^-1	0.6-60	[29, 30]
HipB binds to promoter	P + B₂	P'	θ_B2	1500	hour^-1/mol	150-15,000	approximated based on [31]
HipB unbinds	P'	P + B₂	γ_B2	60	hour^-1	6-600	approximated based on [31]
HipB-HipA complex binds to promoter	P + AB₂A	P''	θ_AB2A	1500	hour^-1/mol	150-15,000	[31]
HipBA unbinds	P''	P + AB₂A	γ_AB2A	60	hour^-1	6-600	[31]
mRNA degradation	M	0	δ_M	6	hour^-1	0.6-60	[32]
HipB translation	M	M + B	β_B	60	mol/hr/mRNA	6-600	[29, 30]
HipA translation	M	M + A	β_A	12	mol/hr/mRNA	1.2-120	[29, 30]
HipB degradation	B	0	δ_B	18	hour^-1	1.8-180	[21]
HipA degradation	A	0	δ_A	1.2	hour^-1	0.12-12	[31, 32]
HipB dimerization	B + B	B₂	β_B2	60	hour^-1	6-600	assumed monomeric form to be uncommon
HipB dimer degradation	B₂	0	δ_B2	5	hour^-1	0.5-50	[21]
HipB-HipA complex association	B₂ + 2A	AB₂A	μ	60	hour^-1/mol	6-600	approximated based on [31]
HipB-HipA complex dissociation	AB₂A	B₂ + 2A	μ_R	60	hour^-1	6-600	approximated based on [31]
HipB-HipA complex degradation	AB₂A	0	δ_AB2A	1.2	hour^-1	0.12-12	[31, 32]

ISSN: 1752-0509