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Figure 2 | BMC Systems Biology

Figure 2

From: A quantitative model of the initiation of DNA replication in Saccharomyces cerevisiae predicts the effects of system perturbations

Figure 2

Example of  in vivo  timecourse experiment. (A) Western blot probed with α-Myc antibody to detect the Cdc6-Myc fusion protein. The corresponding Ponceau-S membrane stains are shown; these serve as loading controls to which densitometric readings were normalized. The labels indicate the time (min) elapsed since release from α-factor; S and P denote the supernatant (soluble protein) and pellet (DNA-bound) fractions, respectively. (B) FACS analysis of the samples described in A, along with an asynchronous culture sample (Async) prior to α-factor arrest.

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