Example of in vivo timecourse experiment. (A) Western blot probed with α-Myc antibody to detect the Cdc6-Myc fusion protein. The corresponding Ponceau-S membrane stains are shown; these serve as loading controls to which densitometric readings were normalized. The labels indicate the time (min) elapsed since release from α-factor; S and P denote the supernatant (soluble protein) and pellet (DNA-bound) fractions, respectively. (B) FACS analysis of the samples described in A, along with an asynchronous culture sample (Async) prior to α-factor arrest.