Figure 4From: Different designs of kinase-phosphatase interactions and phosphatase sequestration shapes the robustness and signal flow in the MAPK cascadeAn implicit negative feedback loop from Phos2 to MKKK in the system M4K1. (A) Kinetics of MKKK-P in M4K1 (M4 with Michaelis Menten kinetics) for a low (5 nM) and a high (1000 nM) Phos2 concentration is shown for unsequestrated (USEQ) condition. and phosphatase sequestrated (PSEQ) conditions. MKKK-P phosphorylation kinetics for low and high Phos2 concentrations is given by black and red dotted lines respectively. (B) Kinetics of MKKK-P in M4K1 (M4 with Michaelis Menten kinetics) for a low (5 nM) and a high (1000 nM) Phos2 concentration is shown for phosphatase sequestrated (USEQ) condition. (C) Steady state phosphorylation amplitude of MKKK-P at various concentrations of Phos2 is shown. Steady state MKKK-P amplitude in PSEQ and USEQ conditions are given with blue and green dotted lines.Back to article page