Figure 6
Strength of the implicit negative feedback from Phos2 to MKKK layer is determined by the extent of sequestration in the MAPK cascade. (A) Steady state MKKK-P amplitude at two different Phos2 concentrations: low (5 nM) and high (1000 nM) when sequestration strength was varied is shown. The plot displays the results for the model M4K1. (B) Steady state MKKK-P amplitude at two different Phos2 concentrations: low (5 nM) and high (1000 nM) when sequestration strength was varied is shown. The plot shows the results for the model M4K2. (C) Schematics demonstrating the decrease in feedback strength with increase in extent of sequestration which is true for both M4K1 and M4K2. The sequestration strength is shown increasing from left to right schematics. The red bar representing the implicit negative feedback from Phos2 to MKKK layer is thickest in USEQ condition and with increase in phosphatase sequestration strength the thickness of the red bar is gradually shown decreasing.