Characterizing criticality of proteins by systems dynamics: Escherichia coli central carbon metabolism as a working example

Table 1 Comparison with multiple E. coli gene essentiality studies

Protein	Gene	Criticality	Flux-sum	Keio collection	Genetic footprinting	PEC database
PGK	pgk	Critical	√	√	√
GAPDH	gapA	Critical	√	√	√
ENO	eno	Critical	√	√		√
ALDO	fbaA	Critical	√	√	√	√
PGI	pgi	Uncritical	√	√	√	√
PK	pykF	Uncritical	√	√	√	√
PGM	pgm	Uncritical	√	√	√	√
Ru5P	rpe	Critical	√		√
G1PAT	glgC	Uncritical	√	√	√	√

Our characterizations of protein criticality (Col 3) are compared with multiple gene essentiality studies (Col 5-7) besides the validations through flux-sum (Col 4).
Note: "√" means this evidence supports the characterization of criticality

ISSN: 1752-0509