Table 1 Alignment rotation error. Subtomogram alignment error in terms of the difference in the determined and true rotational angle of the subtomograms. Shown are the medians and median absolute deviations of all 100 subtomogram alignments. Bold font shows all the alignments with errors larger than 5°, which are considered inaccurate.

±90°

0.71 ± 0.49

3.3 ± 2.8

2.6 ± 1.4

14 ± 9.3

0.89 ± 0.54

2.6 ± 2.1

2.4 ± 1.1

8.5 ± 4.5

±80°

0.85 ± 0.54

2.5 ± 1.8

3.5 ± 2.4

21 ± 14

1.1 ± 0.61

2.2 ± 1.6

3.2 ± 2.2

12 ± 7.7

±70°

1.2 ± 0.53

1.9 ± 1.3

3.1 ± 1.7

19 ± 12

2 ± 0.86

2.1 ± 1

2.9 ± 1.3

16 ± 11

±60°

0.97 ± 0.49

2 ± 0.97

3.7 ± 2.4

49 ± 45

1.5 ± 0.82

2.4 ± 1.2

3.8 ± 2.1

34 ± 30

±50°

1.8 ± 0.9

2.9 ± 1.6

7 ± 5.2

87 ± 63

2.6 ± 1.1

3.4 ± 1.8

6.3 ± 4.2

43 ± 37

±40°

1.6 ± 1

9 ± 8.3

55 ± 53

123 ± 31

15 ± 14

92 ± 40

106 ± 37

113 ± 26

∞

1

0.5

0.1

∞

1

0.5

1

Tilt

±90°

0.58 ± 0.25

1.1 ± 0.57

2 ± 0.84

8.1 ± 2.7

0.7 ± 0.34

1.1 ± 0.47

1.7 ± 0.77

5.9 ± 2.4

±80°

0.79 ± 0.31

1.4 ± 0.6

2.4 ± 0.93

11 ± 4.5

1.2 ± 0.49

1.7 ± 0.73

2.3 ± 0.97

7.9 ± 3

±70°

1 ± 0.26

1.8 ± 0.69

2.7 ± 1.2

8.4 ± 3.1

1.5 ± 0.46

2.1 ± 0.7

2.5 ± 1.1

7.9 ± 2.5

±60°

1 ± 0.42

1.6 ± 0.66

2.7 ± 0.86

10 ± 4.8

1.7 ± 0.68

2.1 ± 0.84

2.8 ± 0.84

9.2 ± 4.5

±50°

2 ± 0.77

2.4 ± 0.93

2.7 ± 1

14 ± 11

2.6 ± 1.1

2.9 ± 1.1

2.9 ± 1.1

11 ± 5.1

±40°

1.5 ± 0.79

2.5 ± 1.1

5.7 ± 3.6

107 ± 27

9.4 ± 7.8

5.7 ± 3.3

7.7 ± 5.4

111 ± 19