From: High precision alignment of cryo-electron subtomograms through gradient-based parallel optimization
60° angle interval, RCS | |||||
---|---|---|---|---|---|
 | SNR | ∞ | 1 | 0.5 | 0.1 |
Tilt | Â | ||||
±90° |  | 0.035 ± 0.023 | 0.16 ± 0.12 | 0.19 ± 0.12 | 0.96 ± 0.66 |
±80° |  | 0.045 ± 0.029 | 0.24 ± 0.2 | 0.21 ± 0.15 | 1.3 ± 0.89 |
±70° |  | 0.078 ± 0.037 | 0.25 ± 0.17 | 0.3 ± 0.18 | 1.3 ± 0.74 |
±60° |  | 0.068 ± 0.036 | 0.19 ± 0.12 | 0.43 ± 0.3 | 2.2 ± 1.3 |
±50° |  | 0.14 ± 0.078 | 0.26 ± 0.17 | 0.65 ± 0.51 | 2.3 ± 1.3 |
±40° |  | 0.15 ± 0.092 | 0.74 ± 0.64 | 1.7 ± 1.3 | 3.2 ± 1.6 |
60° angle interval, FCS | |||||
 | SNR | ∞ | 1 | 0.5 | 0.1 |
Tilt | Â | ||||
±90° |  | 0.047 ± 0.023 | 0.12 ± 0.081 | 0.11 ± 0.053 | 0.49 ± 0.31 |
±80° |  | 0.053 ± 0.03 | 0.15 ± 0.1 | 0.18 ± 0.1 | 0.85 ± 0.66 |
±70° |  | 0.11 ± 0.057 | 0.13 ± 0.074 | 0.21 ± 0.1 | 0.95 ± 0.58 |
±60° |  | 0.11 ± 0.061 | 0.2 ± 0.094 | 0.3 ± 0.15 | 1.6 ± 1.2 |
±50° |  | 0.19 ± 0.1 | 0.28 ± 0.16 | 0.44 ± 0.26 | 1.8 ± 1.2 |
±40° |  | 0.61 ± 0.54 | 3.3 ± 2.7 | 4.3 ± 2.6 | 6.2 ± 3 |
45° angle interval, RCS | |||||
 | SNR | ∞ | 1 | 0.5 | 0.1 |
Tilt | Â | ||||
±90° |  | 0.031 ± 0.014 | 0.072 ± 0.031 | 0.12 ± 0.049 | 0.43 ± 0.21 |
±80° |  | 0.051 ± 0.027 | 0.11 ± 0.051 | 0.17 ± 0.072 | 0.69 ± 0.32 |
±70° |  | 0.063 ± 0.024 | 0.14 ± 0.052 | 0.21 ± 0.1 | 0.63 ± 0.24 |
±60° |  | 0.076 ± 0.036 | 0.15 ± 0.068 | 0.23 ± 0.1 | 0.89 ± 0.5 |
±50° |  | 0.11 ± 0.055 | 0.2 ± 0.094 | 0.28 ± 0.14 | 1.3 ± 0.95 |
±40° |  | 0.14 ± 0.071 | 0.31 ± 0.17 | 0.67 ± 0.47 | 6.2 ± 5.3 |
45° angle interval, FCS | |||||
 | SNR | ∞ | 1 | 0.5 | 0.1 |
Tilt | Â | ||||
±90° |  | 0.033 ± 0.016 | 0.071 ± 0.03 | 0.094 ± 0.032 | 0.29 ± 0.13 |
±80° |  | 0.061 ± 0.031 | 0.1 ± 0.052 | 0.13 ± 0.062 | 0.46 ± 0.21 |
±70° |  | 0.08 ± 0.04 | 0.12 ± 0.05 | 0.17 ± 0.075 | 0.49 ± 0.22 |
±60° |  | 0.1 ± 0.052 | 0.17 ± 0.091 | 0.22 ± 0.073 | 0.72 ± 0.36 |
±50° |  | 0.19 ± 0.094 | 0.22 ± 0.083 | 0.24 ± 0.12 | 0.93 ± 0.5 |
±40° |  | 0.76 ± 0.64 | 0.51 ± 0.36 | 0.82 ± 0.6 | 9.8 ± 4.1 |