Skip to main content

Table 4 Constrained alignment translation error. Constraining the search to rotations around a single axis. Subtomogram alignment error in terms of the difference in the Euclidean distance between determined and true subtomogram translations. Shown are the medians and median absolute deviations of all 100 subtomogram alignments.

From: High precision alignment of cryo-electron subtomograms through gradient-based parallel optimization

60° angle interval, RCS

 

SNR

1

0.5

1

Tilt

 

±90°

 

0.018 ± 0.0053

0.047 ± 0.017

0.08 ± 0.023

0.28 ± 0.1

±80°

 

0.027 ± 0.011

0.069 ± 0.029

0.1 ± 0.048

0.37 ± 0.17

±70°

 

0.037 ± 0.017

0.085 ± 0.04

0.13 ± 0.059

0.45 ± 0.28

± 60°

 

0.055 ± 0.028

0.14 ± 0.083

0.19 ± 0.11

0.59 ± 0.36

±50°

 

0.1 ± 0.067

0.18 ± 0.12

0.24 ± 0.12

0.74 ± 0.42

±40°

 

0.27 ± 0.25

0.49 ± 0.4

0.94 ± 0.83

3.3 ± 2.3

60° angle interval, FCS

 

SNR

1

0.5

0.1

Tilt

 

±90°

 

0.018 ± 0.0062

0.05 ± 0.017

0.074 ± 0.021

0.23 ± 0.097

±80°

 

0.027 ± 0.013

0.063 ± 0.025

0.076 ± 0.029

0.24 ± 0.091

±70°

 

0.034 ± 0.015

0.077 ± 0.032

0.098 ± 0.036

0.32 ± 0.16

±60°

 

0.055 ± 0.033

0.12 ± 0.063

0.18 ± 0.099

0.46 ± 0.28

±50°

 

0.11 ± 0.075

0.16 ± 0.099

0.2 ± 0.11

0.6 ± 0.36

±40°

 

0.39 ± 0.35

0.36 ± 0.27

0.57 ± 0.44

3.5 ± 2.8

45° angle interval, RCS

 

SNR

1

0.5

0.1

Tilt

 

±90°

 

0.015 ± 0.005

0.054 ± 0.014

0.063 ± 0.019

0.24 ± 0.086

±80°

 

0.024 ± 0.0072

0.056 ± 0.018

0.086 ± 0.028

0.29 ± 0.12

±70°

 

0.033 ± 0.0095

0.075 ± 0.028

0.11 ± 0.04

0.38 ± 0.17

±60°

 

0.046 ± 0.019

0.1 ± 0.036

0.16 ± 0.051

0.48 ± 0.22

±50°

 

0.067 ± 0.035

0.14 ± 0.06

0.22 ± 0.098

0.59 ± 0.27

±40°

 

0.22 ± 0.18

0.31 ± 0.21

0.44 ± 0.29

2.3 ± 1.7

45° angle interval, FCS

 

SNR

1

0.5

0.1

Tilt

 

±90°

 

0.017 ± 0.0053

0.048 ± 0.014

0.06 ± 0.016

0.21 ± 0.074

±80°

 

0.021 ± 0.0069

0.052 ± 0.018

0.073 ± 0.025

0.2 ± 0.067

±70°

 

0.03 ± 0.011

0.065 ± 0.023

0.098 ± 0.03

0.26 ± 0.085

±60°

 

0.043 ± 0.017

0.088 ± 0.033

0.13 ± 0.044

0.32 ± 0.11

±50°

 

0.07 ± 0.032

0.14 ± 0.056

0.18 ± 0.073

0.44 ± 0.19

±40°

 

0.17 ± 0.11

0.24 ± 0.16

0.33 ± 0.18

1.5 ± 1.2