Cellular localization of the miRNAs in EHBDs. In situ hybridization performed on EHBDs from 7-day old neonatal mice reveals substantial expression of the miRNAs in cholangiocytes (arrows) and stromal cells in the subepithelial compartment (arrowheads). Probe for U6 microRNA served as positive control and scramble probe served as negative control. Asterisks indicate the bile duct lumen. Scale bar: 50 μm.