Figure 5

Topological properties of refactored genomes. (A) Complexity reduction (number of regulatory interactions and operons are represented using blue and red bars, respectively) of the refactored genomes with respect to the wild-type genome under permissive environments using an evolutionary process including tandem promoters (dark bars) or genetic re-organization of E. coli transcriptional units only (light bars). (B) Number of genes vs operons regulated by a given wild-type promoter (red points) or a tandem promoter added to the synthetic transcription unit (blue points), as proposed for the refactored genomes evolved using selective pressure based only on genes coding for enzymatic activity (squares), genes related to adaptation and defense functions (triangles) or the entire genome (circles). Error bars represent standard deviations of scores obtained from 10 independent.