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Figure 2 | BMC Systems Biology

Figure 2

From: Systematic design methodology for robust genetic transistors based on I/O specifications via promoter-RBS libraries

Figure 2

The amplifier design example of synthetic genetic transistor. For amplifier design example of synthetic genetic transistor, the prescribed amplification gain = -2 within the range g1 [298, 431] is computed by (11) (red line). The most adequate promoter-RBS component c3 and aTc concentration I aTc to fit the prescribed amplification gain are searched as {c3, I aTc } = {T3, 0 ng/ml} by minimizing J A (c3, I aTc ) in (16) from the corresponding promoter-RBS library Lib Tet and concentration range of inducer I aTc . The green points are the experimental results based on {c3, I aTc }, and the error bars are the standard deviations. The green line is the estimation of I/O response of the synthetic genetic transistor based on experimental data, with the estimated amplification gain = -1.978. Obviously, the amplification gain of the designed genetic transistor could match the desired amplification gain quite well.

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