Workflow of AKI sample analysis. Systems Biology approaches require prior knowledge and/or inferred pathways from independent sources used to map measured data (top). In this work these sources include an extensive novel proteomic dataset, and that has undergone rigorous statistical analysis. The predicted affected pathways are then mapped further by extensive manual literature mining and delineation to establish plausible cascades and pathway maps, followed by integration of all data to assemble a final model. Re-iterations between each and all steps between pathway mapping and model assembly ensure a best-fit model for both the de-novo data as well as prior knowledge.