In silico knock-out of PEMT/PLMT (R9). The bar plots illustrate the results of in silico knock-out of PEMT/PLMT in presence of SerE (100 μ M) and in absence of ChoE, EtnE. ‘BKO’ denotes before knockout and ‘AKO’ denotes after knockout. The upper panel shows the effect on effluxes, whereas the lower panel shows the effect on concentrations of end products. The fluxes and concentrations were calculated by simulating the ODE (PL) model for two hours starting from zero initial metabolite concentrations.