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Table 2 Parameter values and confidence intervals for the ODE (PL) model

From: Kinetic modelling of phospholipid synthesis in Plasmodium knowlesi unravels crucial steps and relative importance of multiple pathways

Reaction ID Reactions Enzymes Estimated Vm( μ M/min) Estimated Km ( μ M) Regime
R1 SerE → Ser .. 3.41 [ 3.08,3.95] 363 [ 326,410]  
R2 Ser → PS PSS 1.31 [ 0.517,1.65] 797 [ 352,1.25.103]  
R3 Ser → Etn SD 2.62 [ 2.33,3.11] 24 [ 22.7,29.5]  
R4 Etn → PEtn EK 8.62 [ 6.19,13.3] 109 [ 75.6,193]  
R5 PEtn → PCho PMT 1.08 [ 0.725,13.9] 122 [ 78.5,2650]  
R6 PS → PE PSD 2.25 [ 0.747,2.94] 204 [ 152,413]  
R7 PEtn → PE ECT/CEPT 5.61 [ 4.19,7.65] 227 [ 175,353]  
R8 PCho → PC CCT/CEPT 0.413 [ 0.315,0.599] 31 [ 26.2,34.9]  
R9 PE → PC PEMT/PLMT 1.42.103 [ 59.2,1.33.104] 3.21.105 [ 2.73.104,1.92.106] L
R10 PC → PS PSSbe 0.697 [ 0.318,0.944] 3.76 [ 0.06,3.88]  
R11 PE → PS PSSbe 89.9 [ 9.16,527] 1.71.105 [ 1.58.104,9.14.105] L
R12 PC → .. 1.57 [ 0.75,3.84] 24.1 [ 16.5,35.8]  
R13 PS → .. 1.54 [ 0.013,3.28] 204 [ 41.1,1020]  
R14 PE → .. 774 [ 9.44,1.08.104] 1.55.105 [ 2.52.104,1.65.106] L
R15 ChoE → Cho OCT/NPP 0.232 [ 0.182,0.323] 102 [ 91.9,109]  
R16 Cho → PCho CK 0.556 [ 0.384,0.882] 30.4 [ 22.8,36.7]  
R17 EtnE → Etn .. K f =5.10−4m i n−1 [ 10−4,2.5.10−3] K b =1.33.10−4m i n−1 [ 4.45.10−15,1.4.10−3]  
  1. For the calculation of the confidence intervals see the Methods section and the Additional file 1 (the allowed objective function values were less than 1.5 times the global minimum). All the reactions have mass action kinetics, with the exception of the reaction 17 that has first order mass action law kinetics (the forward and the backward rate constants are denoted K f and K b , respectively). For those reactions whose regime is linear (L), only the ratio V max /K m is significant.
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