Figure 1

Workflow of the method used in this study. The figure represents the pipeline that has been followed in this study to identify essential genes. Four different methods have been applied independently to filter probable candidates for essentiality. The results thus obtained have been integrated using Boolean logic to obtain a final list of essential genes. In addition, integrated scores have also been assigned to each gene. The numbers in the bracket represents the initial number of genes analysed using the different methods, which are further filtered.