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Figure 6 | BMC Systems Biology

Figure 6

From: Carbon catabolite repression correlates with the maintenance of near invariant molecular crowding in proliferating E. coli cells

Figure 6

Cell growth and substrate consumption are inhibited by inducers in batch culture of MG1655 cells in mixed substrate. Overnight LB cultures of E. coli cells were inoculated into 125 ml flask with 15 ml M9 media with 0.2% mixed substrate. The initial OD600 was ~0.03 ~ 0.04 and cells were cultured for 6 hr on a shaker with 200 rpm at 37°C. The inducers, 4 mM cAMP and 200 μM maltotriose (++) were added after 15 min culture. Cell growth (A) and growth rate (B) were sampled every 2 hr and substrate consumption rate of glucose, maltose and glycerol were determined (C). Maltose transporter MBP (MalE) level (the inset showing the immunostaining result) (D) and cell density (E) were measured at 4th and 6th hr by Ficoll gradient and western blot respectively. The protein expression level in (D) was determined with ImageJ. Cell volume (F) was measured by Beckman Coulter.

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