Figure 5

Six cellular transitions corresponding to six illustrative biological examples are represented in this figure after the perturbation of specific minimal combinations of reprogramming determinant genes. Simulated perturbations performed assuming a Boolean model succeeded in triggering the transition. These results are consistent with experimental perturbations previously published. Genes in “ON” and “OFF” are represented in grey and white respectively; these states correspond to the characteristic expression profile in both initial and final states. Points of perturbation of DEPCs are marked with red arrow. These genes should change their states in order to induce the desired cellular transition. a) T-helper. Perturbation of T-BET induces the transition from Th2 to Th1. b) EMT. Perturbation of SNAI1 induces de transition from epithelial to mesenchymal c) HL60. Perturbation of IRF1 induces the transition from HL60 to neutrophils. d) iHEP. Combined perturbation of HNF4 and FOXA2 induce the transition from fibroblast to hepatocyte. e) ICM. Perturbation of GATA4 induces the transition from fibroblast to cardiomyocyte. f) iPSCS. Combined perturbation of SOX2, POU5F1 and TP53 induce the transition from fibroblast to iPSCS. Regular and T-arrows represent activation and inhibition respectively.