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Table 2 Physiological characteristics of the strains and conditions studied

From: Mapping global effects of the anti-sigma factor MucA in Pseudomonas fluorescens SBW25 through genome-scale metabolic modeling

Carbon source Strain OD 660 C source Uptake [mmolC/ gDW h] Alginate production, deacetylated [mmolC/gDW h] Acetate (acetyl from alginate) [mmolC/gDW h] CO 2 excretion [mmolC/ gDW h] C in biomass [mmolC/ gDW h] C balance [%]
Fructose Wild-type 7,8 9,4 - - 7,5 1,51 95,7
Fructose mucA 7,0 18,3 12,3 0,77 4,6 1,51 104,4
Fructose ΔalgC 8,1 9,1 - - 7,4 1,51 97,6
Fructose mucA ΔalgC 8,4 5,8 - - 4,3 1,51 99,3
Fructose mucA TTalgD 9,2 5,6 - - 3,9 1,51 96,9
Glycerol SBW25 9,2 8,3 - - 6,4 1,51 96,3
Glycerol mucA 7,4 17,5 10,6 1,22 3,6 1,51 96,8
Glycerol mucA TTalgD 9,9 6,9 - - 5,0 1,51 95,4
  1. Measured and calculated physiological parameters for chemostat cultivations (D = 0.04) of P. fluorescens SBW25 (wild-type) and derived mutant strains. The value for mmolC/gDW h in biomass is calculated from the specific growth rate used in the experiments (μ = 0.04 hr-1) and the biomass stoichiometric composition assumed in the genome scale model. The % value for carbon balance represents the sum of carbon accounted for in alginate, CO2 and biomass as a percentage of the carbon taken up by the cells.